Spectrophotometric Determination of Captopril and Penicillamine through the Use of Ligand Exchange Complexation Reactions.

Two spectrophotmetric methods based on combined redox - ligand exchange reactions were developed for the determination of captopril and penicillamine in pure form and in their dosage forms. The first method is based on attenuating the absorbance of a ternary complex: silver (I) - bromopyrogallol red - phenanthroline in a buffer solution of pH6-8. The method has the concentration ranges 2-10 μg mL(-1) and 0.5-1.75 μg mL(-1) for captopril and penicillamine respectively, and the detection limits 7.1 × 10(-2) and 5.7 × 10(-2) μg mL(-1) for captopril and penicillamine respectively. The second method is based on decreasing the absorbance of a chloroformic solution of copper (II) - oxine chelate when shaken with the drug solution in buffer medium of pH 8. The drugs were determined in the concentrations 30-90 μg mL(-1) and 30-100 μg mL(-1) for captopril and penicillamine respectively, and the detection limits 0.94 and 1.76 μg mL(-1) for captopril and penicillamine respectively. The proposed methods were applied in the analysis of both compounds in their pharmaceutical preparations, and results were favorably compared with reference spectrophotometric methods regarding accuracy and precision.

Viewing the reported spectrophotometric methods for determining CAP and PEN, revealed that the use of ligand exchange reactions using ternary or binary complexes was hitherto not reported.
The interactions of CAP and PEN with Ag(I) -Bromopyrogallol red -phenanthroline, or Copper (II) oxinate (CuQ 2 ) chloroformic solution were developed into two spectrophotometric methods for their determination in their pure state and in their pharmaceutical preparations. The proposed methods proved to be simple and of accuracy and precision that are comparable to previously published methods, and selective for the two thiol containing drugs in presence of their disulphide oxidation products and common excipients of their pharmaceutical preparations.

EXPErIMENtAL Instrumentation
The instrument used was Perkin-Elmer Lambda EZ201 UV-visible spectrophotometer with matched 1 cm quartz cells.

reagents and solutions
Drug solutions: Stock solution of each drug was prepared in distilled water to give 0.5 mg mL -1 and 40 µg mL -1 for CAP and PEN (Sigma Chem Co, Milwaukee-WI, USA), respectively for use in method I. Standard solutions containing 0.5 mg mL -1 and 40 µg mL -1 for PEN and CAP, respectively were prepared for use in method II. reagents for Method I.
• Copper quinolin-8-olate (CuQ 2 ) (prepared by the gravimetric method (35)), by dissolving 60 mg of the solid reagent in 1 L of chloroform (Adjust the concentration to exhibit absorbance of about 1.0 at λ max of 410 nm. • Acetate buffer solutions of pH5-8.

General procedure and construction of calibration graphs
Method I. Volumes of 0.2-1.1 mL of the standard solution were transferred to a set of 25 mL volumetric flasks, and the volume was completed to 1.1 mL with distilled water. Volumes of 1 mL 20% ammonium acetate, 0.5 mL gelatin solution, 0.5 mL of Phen, 2 mL of silver nitrate and finally 1 mL BPR were added to each flask. Solutions were mixed, completed to volume with distilled water and allowed to stand for 5 minutes at room temperature. A blank experiment was carried out by omitting CAP or PEN. Absorbance of the blank solution was measured against the sample solution at λ max of 635 nm.
Application to cAP tablets. Twenty tablets were powdered and a quantity of the powder equivalent to 25 mg CAP were extracted with 25 mL of distilled water in a 50 mL volumetric flask and completed to volume with distilled water. The suspension was filtered and an aliquot volume of the filtrate was used for analysis as described under Method I.
Application to PEN capsules. The contents of 20 capsules were mixed and a weighed quantity of the mixed contents equivalent to 4 mg of PEN was transferred into a 100 mL volumetric flask containing 30 mL distilled water. The mixture was shaken mechanically for 10 minutes, completed to volume with distilled water, filtered and an aliquot volume of the filtrate was used for analysis as described under Method I.
Method II. Into 60 mL separating funnel, accurate volumes of standard solutions in the concentration range of 30-100 µg mL -1 for CAP and PEN were measured. The volumes were completed to 5 mL with distilled water and the pH of the solutions adjusted to pH 6 using pH paper. A volume of 10 mL of copper quinolin-8-olate solution was added and the mixture was shaken for 5 minutes with 10 mL of chloroform. The organic solvent layer was separated and dried over anhydrous sodium sulphate and the absorbance of the blank solution was measured against the sample at λ max of 410 nm.
Procedure for tablets and capsules. Twenty tablets were weighted and powder or the contents of 20 capsules were mixed, and an accurately weighed quantity equiva-lent to 25 mg CAP or PEN was transferred into 50 mL volumetric flask half filled with distilled water, shaken for 15 minutes and diluted with the same solvent. The solution was filtered and first portion was discarded. Volumes of the filtrate were transferred into a series of 60 mL separators funnels and completed as described under Method II.

Method I
The ternary complex ([Ag(I) (Phen)] 2 BPR) was first reported by Dagnal & West (36). We found that when CAP or PEN was added to this complex, a silver mercaptide of the drug is formed, and the colour of the ternary complex is decreased proportionally. Figure 2 illustrates the absorption spectra of the ternary complex in presence and absence of the investigated drugs at the same λ max where the addition of the drugs caused only decrease in absorbance.

Optimum reaction conditions
• The effect of pH was studied over pH range of 4-10.
The best results were obtained in the range of pH 6 -8 using a solution of 2% w/v solution of ammonium acetate (Figure 3). • The effect of reagent concentration: The ternary complex formation was optimized by using at least 4-fold molar excess of BPR and Phen, respectively over Ag(I). Two and half mL of 25 µg mL -1 of silver nitrate were suitable for CAP and PEN (Figure 4). • Selection of stabilizing agent to avoid precipitation of silver ternary complex. Among Tritox X-100, sodium lauryl sulphate, gelatin solutions, a 0.5% gelatin solution was the best stabilizer which acts as a protective colloid. • The stoichiometry of the reaction: The composition of the ternary complex was reported to be [Ag(I) (Phen) 2 , BPR] and its reaction with CAP and PEN as determined using continuous variation method (37) was found to be 1:1 (Drug to ternary complex). Table 1 shows the range of linearity and statistical analysis at λ max of 635 nm on using method I.    accuracy and precision. The small RSD% and SAE indicate high precision and accuracy. Application to tablets and capsules: The results of applying method I to analyse CAP and PEN in dosage forms and comparison with reference methods as shown in Table 3 indicates that fairly comparable results of developed method with reference methods (38) by applying statistical analysis.

Method II
8-hydroxyquinoline (oxine) forms coloured chelates with metal ions including copper (II) (39) which are extractable with chloroform (CuQ 2 ). In this method we found that when the chloroformic Cu(II)-oxine solutions was shaken with CAP or PEN, a decrease in absorbance of the chelate at λ max of 410 nm occurred which was proportional to the drug added. Figure 5 shows the absorption spectrum of CuQ 2 in presence of CAP or PEN.
For CAP, it may be assumed that it reduces Cu(II) to Cu(I) and CAP itself is oxidized to its disulphide (40). For PEN, a coupled redox complexation reaction takes place in which the disulphide formed gives Cu(I)-PEN chelate (41), and a decrease in absorbance of CuQ 2 happens.
Calibration curves and statistical analysis of results of the use of method II, the linearity range, correlation coefficients and variances are shown in Table 4.  Accuracy and precision are illustrated in Table 5 indicating high precision and accuracy (low RSD% and SAE).
The proposed method (II) was applied to both CAP and PEN in dosage forms and was compared with a reference method in Table 6. Comparable results obtained by both methods by students' t test and variance ratio F test.

Interference study in methods I and II
It was found that common tablets and capsules excipients as well as oxidized drugs (disulphide) did not interfere in both procedures. Hydrochlorothiazide (commonly prescribed with CAP) is insoluble in water; consequently it does not interfere. The proposed procedures are recommended as simple method in quality control laboratories for the analysis of CAP or PEN in the pure state and dosage forms.

comparison between methods I and II
Method I is of higher sensitivity than method II. Regarding accuracy and precision, both methods are equivalent, and results of both methods are comparable to the reference spectrophotometric methods applied simultaneously. Method I is relatively advantageous to method II because of the ease by which solutions were prepared.

cONcLUsION
Two simple spectrophotometric methods based on application of ligand exchange reactions were presented. The described methods were applied on two thiol group containing compounds: captopril and penicillamine. No interference could be observed from the disulphide degradation products of the two compounds. The applicability of the developed methods was evaluated through the determination of the two drugs in bulk form and in several pharmaceutical formulations with good accuracy and precision. rEFErENcEs